4GWU
Crystal Structure of Fru 2,6-bisphosphate complexes of Porcine Liver Fructose-1,6-bisphosphatase with Filled Central Cavity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-12 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.999, 81.221, 165.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.539 - 3.000 |
| R-factor | 0.23382 |
| Rwork | 0.231 |
| R-free | 0.28869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yyz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.683 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.539 | 38.539 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.450 | 3.000 |
| Rmerge | 0.163 | 0.068 | 0.516 |
| Number of reflections | 7478 | ||
| <I/σ(I)> | 4.8 | 11 | 1.6 |
| Completeness [%] | 98.4 | 94.4 | 99.9 |
| Redundancy | 3.48 | 3.42 | 3.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |
