4GWO
Crystal structure of ligand binding domain of LysR family member,CysB in complex with sulfate from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.921, 105.301, 47.751 |
| Unit cell angles | 90.00, 103.26, 90.00 |
Refinement procedure
| Resolution | 26.325 - 2.394 |
| R-factor | 0.2019 |
| Rwork | 0.198 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1al3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.710 |
| High resolution limit [Å] | 2.394 |
| Rmerge | 0.034 |
| Number of reflections | 17828 |
| <I/σ(I)> | 38.14 |
| Completeness [%] | 67.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30%(w/v) PEG5000 MME,0.2M ammonium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
