4GW9

Structure of a bacteriophytochrome and light-stimulated protomer swapping with a gene repressor

Replaces:  4EHO

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-02-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.97911, 0.97949, 0.98244, 0.9700
Spacegroup name	P 1 21 1
Unit cell lengths	102.940, 146.870, 139.550
Unit cell angles	90.00, 101.17, 90.00

Refinement procedure

Resolution	15.000 - 2.900
R-factor	0.20169
Rwork	0.200
R-free	0.24318
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	2.523
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	97789
<I/σ(I)>	9.6	1.4
Completeness [%]	97.7	96.4
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	PROTEIN CONCENTRATION 20 MG/ML, 4% POLY-GAMMA-GLUTAMIC ACID POLYMER, 100 MM TRISHCL PH 8, 0.4 M NIACINAMIDE, 200 MM KBR, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	8	292	PROTEIN CONCENTRATION 20 MG/ML, 4% POLY-GAMMA-GLUTAMIC ACID POLYMER, 100 MM TRISHCL PH 8, 0.4 M NIACINAMIDE, 200 MM KBR, VAPOR DIFFUSION, SITTING DROP, temperature 292K