4GW3
Crystal Structure of the Lipase from Proteus mirabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-16 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.502 |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.438, 65.438, 63.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.130 - 2.000 |
| R-factor | 0.1686 |
| Rwork | 0.167 |
| R-free | 0.19420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.871 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.137 | 0.080 | 0.430 |
| Number of reflections | 20166 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 97.7 | 99.3 | 96.2 |
| Redundancy | 3.8 | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5.6 | 298 | 0.095 M Sodium citrate tribasic dihydrate pH 5.6, 19% v/v 2-Propanol, 19% w/v PEG 4000, 5% glycerol, hanging drop, temperature 298K |
