4GVT
Crystal structure of D48V mutant of human GLTP bound with 12:0 disulfatide (hexagonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9754 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 111.019, 111.019, 75.206 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.810 - 2.900 |
| R-factor | 0.19771 |
| Rwork | 0.193 |
| R-free | 0.28201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h2z |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.020 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 6872 | |
| Completeness [%] | 96.5 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10-20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
