4GVO
Putative L-Cystine ABC transporter from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.990, 170.628, 45.049 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 31.800 - 1.448 |
| R-factor | 0.10841 |
| Rwork | 0.106 |
| R-free | 0.15355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1m |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.543 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.480 |
| High resolution limit [Å] | 1.448 | 1.448 |
| Rmerge | 0.052 | 0.177 |
| Number of reflections | 76793 | |
| <I/σ(I)> | 17.7 | 4.31 |
| Completeness [%] | 90.7 | 51.5 |
| Redundancy | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M sodium chloride, 20% PEG-3350, 0.01 M histidine , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
