4GVL
Crystal Structure of the GsuK RCK domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 110.648, 161.693, 310.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.450 - 3.000 |
| R-factor | 0.22886 |
| Rwork | 0.228 |
| R-free | 0.26178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1LNQ Gating Ring |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 55435 | |
| <I/σ(I)> | 1.8299 | |
| Completeness [%] | 93.6 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20-23% PEG3350, 120mM KCl, 80mM NaNO3, 1% Glycerol, 100mM Bis-Tris Propane, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
