4GVD
Crystal Structure of T-cell Lymphoma Invasion and Metastasis-1 PDZ in complex with Syndecan1 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.517, 58.106, 50.948 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 50.946 - 1.850 |
| R-factor | 0.19848 |
| Rwork | 0.196 |
| R-free | 0.24302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kzd |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.289 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.946 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.355 |
| Number of reflections | 13246 | |
| <I/σ(I)> | 8.9 | 2.7 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3 | 2.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 0.1 M MES, 20% PEG8000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
