4GVA
ADP-bound form of the ERK2 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Collection date | 2012-06-18 |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.605, 69.072, 59.962 |
| Unit cell angles | 90.00, 108.49, 90.00 |
Refinement procedure
| Resolution | 38.341 - 1.830 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.341 | 38.341 | 1.430 |
| High resolution limit [Å] | 1.360 | 4.290 | 1.360 |
| Rmerge | 0.026 | ||
| Total number of observations | 8645 | 22999 | |
| Number of reflections | 74962 | ||
| <I/σ(I)> | 5.3 | 22.4 | |
| Completeness [%] | 92.2 | 98.2 | 64.3 |
| Redundancy | 3.4 | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop vapor diffusion | 6.5 | 295 | 0.1 M MES pH 6.5, 5% PEG400, 2M ammonium sulfate, sitting drop vapor diffusion, temperature 295K |
