4GUE
Structure of N-terminal kinase domain of RSK2 with flavonoid glycoside quercitrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.084, 40.686, 83.264 |
| Unit cell angles | 90.00, 114.31, 90.00 |
Refinement procedure
| Resolution | 25.293 - 1.800 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20470 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ubd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 28004 | |
| <I/σ(I)> | 15.2 | 4.65 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% Jeffamine ED2003, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
