4GUC
1.4 Angstrom resolution crystal structure of uncharacterized protein BA_2500 from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03316 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.470, 34.708, 61.928 |
| Unit cell angles | 81.33, 79.45, 70.07 |
Refinement procedure
| Resolution | 29.840 - 1.400 |
| R-factor | 0.14932 |
| Rwork | 0.147 |
| R-free | 0.18693 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.772 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.068 | 0.340 |
| Number of reflections | 39510 | |
| <I/σ(I)> | 20.22 | 3.8 |
| Completeness [%] | 95.6 | 92.4 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | Crystallization condition: 25% PEG 3350, 0.2M Na Chloride, 0.1M Bis-Tris pH 5.6. Freezing condition: 25% PEG 3350, 0.2M Na Chloride, 0.1M Bis-Tris 7% Glyserol,7% EG, 7% Sucrose. Protein: 3.4 mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
