Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.750, 122.320, 75.030 |
| Unit cell angles | 90.00, 114.92, 90.00 |
Refinement procedure
| Resolution | 30.580 - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.184 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tez |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.580 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.126 | 0.354 |
| Number of reflections | 44381 | |
| <I/σ(I)> | 11.9 | 1.8 |
| Completeness [%] | 84.0 | 48 |
| Redundancy | 3.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 50 MM HEPES PH 8.0; 150 MM NACL 5MG/ML PROTEIN MIXED 1:1 WITH 18% PDG 4K, 150 MM MGACETATE, 100 MM TRIS PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
