4GTV
Engineered RabGGTase in complex with BMS analogue 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.625, 90.741, 115.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.950 |
| R-factor | 0.17045 |
| Rwork | 0.168 |
| R-free | 0.21143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dss |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.121 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 51134 | |
| <I/σ(I)> | 16.9 | 5.2 |
| Completeness [%] | 98.7 | 98.4 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 285 | RabGGTase in 20mM Hepes pH7.2, 100mM NaCl and 2mM DTT was mixed with 15% PEG3350, 0.2M Ca Acetate, 0.1M Hepes pH7.2. The obtained crystals was soaked with 20% PEG3350, 0.2M Ca Acetate, 5% Glycerol in the presence of 1mM inhibitor for 12 hour, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
