4GTN
Structure of anthranilate phosphoribosyl transferase from acinetobacter baylyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.580, 70.100, 171.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.852 - 2.032 |
| R-factor | 0.1753 |
| Rwork | 0.171 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bpq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.399 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.852 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.060 | 0.621 |
| Number of reflections | 23174 | |
| <I/σ(I)> | 18.7 | 2.1 |
| Completeness [%] | 98.3 | 86 |
| Redundancy | 5.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.45 | 293 | 0.1M Na Cacodylate, 15% PEG8000, 0.2M Mg Acetate, pH 7.45, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
