4GTD
T. Maritima FDTS (E144R mutant) with FAD and dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9809 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.590, 117.390, 142.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.350 - 1.760 |
| R-factor | 0.18123 |
| Rwork | 0.179 |
| R-free | 0.21466 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o26 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.684 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC (5.5.0110) |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.061 | 0.988 |
| Number of reflections | 91492 | |
| <I/σ(I)> | 21.9 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50-60% PEG 200 and 100mM Tris HCL (pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
