4GT4

Structure of unliganded, inactive Ror2 kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	77
Detector technology	CCD
Collection date	2010-11-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	102.834, 112.918, 114.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.800 - 2.406
R-factor	0.1787
Rwork	0.177
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.603
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1108))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.399	2.399
Number of reflections	26279
Completeness [%]	100.0	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	20% PEG3350, 0.2 M magnesium nitrate hexahydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K