4GT3
ATP-bound form of the ERK2 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Collection date | 2012-06-18 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.629, 69.259, 59.816 |
| Unit cell angles | 90.00, 109.03, 90.00 |
Refinement procedure
| Resolution | 38.290 - 1.680 |
| R-factor | 0.1681 |
| Rwork | 0.166 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.292 |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.302 | 1.430 |
| High resolution limit [Å] | 1.357 | 1.357 |
| Rmerge | 0.269 | |
| Total number of observations | 28600 | |
| Number of reflections | 74850 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 92.6 | 73.5 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 0.1 M MES pH 6.5, 5% PEG400, 2M ammonium sulfate, sitting drop vapor diffusion, temperature 295K |
