4GT0

Structure of dengue virus serotype 1 sE containing stem to residue 421

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	200
Detector technology	CCD
Collection date	2011-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999
Spacegroup name	P 63
Unit cell lengths	77.893, 77.893, 292.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.481 - 2.570
R-factor	0.183
Rwork	0.177
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.570	2.570
Rmerge	0.116	0.521
Number of reflections	31819
<I/σ(I)>		1.92
Completeness [%]	99.6	99.1
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K