4GSN
Crystal Structure of GSTe2 ZAN/U variant from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.330, 86.380, 92.850 |
| Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 35923 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 30% PEG 6000, 0.1 M BIsTris pH 6.5, 1 mM B-ME Plates: 24-well VDX plates, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
