4GSB
Monoclinic crystal form of the apo-ERK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Collection date | 2012-03-28 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.780, 69.970, 59.890 |
| Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
| Resolution | 44.020 - 1.800 |
| R-factor | 0.1563 |
| Rwork | 0.154 |
| R-free | 0.19860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ERK |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.132 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.024 | 44.024 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.700 | 1.800 |
| Rmerge | 0.019 | 0.147 | |
| Total number of observations | 3822 | 18554 | |
| Number of reflections | 35077 | ||
| <I/σ(I)> | 16.9 | 31.1 | 4.5 |
| Completeness [%] | 99.3 | 94.4 | 99.4 |
| Redundancy | 3.6 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop vapor diffusion | 6.5 | 295 | 0.1 M MES pH 6.5, 5% PEG400, 2M ammonium sulfate, sitting drop vapor diffusion, temperature 295K |
