4GS4

Structure of the alpha-tubulin acetyltransferase, alpha-TAT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 2
Unit cell lengths	97.931, 130.668, 37.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.797 - 2.112
R-factor	0.2294
Rwork	0.227
R-free	0.25320
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.282
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.800
High resolution limit [Å]	2.100
Number of reflections	26218

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.5	0.1M Tris pH 7.5, 2.7M Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 298.5K