4GRO
Crystallographic and biological characterization of N- and C- terminus mutants of human MIF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-14 |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | H 3 |
| Unit cell lengths | 156.960, 156.960, 96.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.100 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.390 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.078 |
| Number of reflections | 58450 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 98.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
