4GR6

Crystal structure of AtRbcX2 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-05-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	91.770, 63.300, 57.320
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	21.167 - 2.000
R-factor	0.175
Rwork	0.174
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z44
RMSD bond length	0.012
RMSD bond angle	1.206
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1062))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.750
High resolution limit [Å]	2.000
Number of reflections	21808
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	15% PEG 400, 100 mM sodium citrate , pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K