4GR4
Crystal structure of SlgN1deltaAsub
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.95000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.110, 148.610, 109.580 |
| Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
| Resolution | 35.720 - 2.440 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 2.500 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 95771 | |
| <I/σ(I)> | 1.84 | |
| Completeness [%] | 97.2 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 100 mM Tris-HCl, 0.2 M Li2SO4, 1.3 M K/Na tartrate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
