4GR2
Structure of AtRbcX1 from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 45.270, 47.640, 140.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.000 |
| R-factor | 0.2115 |
| Rwork | 0.209 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z44 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1062)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.730 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 20075 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 32% isopropanol, 100 mM MES , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
