4GQW

Crystal structure of CBS-pair protein, CBSX1 (loop deletion) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	55.818, 55.818, 83.003
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.489 - 2.201
R-factor	0.2632
Rwork	0.258
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	9801
<I/σ(I)>	64.8
Completeness [%]	99.9
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1M sodium acetate pH 4.6, 2.0M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K