4GQV

Crystal structure of CBS-pair protein, CBSX1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97923
Spacegroup name	P 31 2 1
Unit cell lengths	56.361, 56.361, 82.595
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.527 - 2.392
R-factor	0.2547
Rwork	0.248
R-free	0.30950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.190
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.392
Number of reflections	11556
<I/σ(I)>	60.8
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	100mM phophate-acetate pH 4.2, 200mM NaCl, 20%(w/w) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 295K