4GQO

2.1 Angstrom resolution crystal structure of uncharacterized protein lmo0859 from Listeria monocytogenes EGD-e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	44.865, 120.033, 221.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.860 - 2.100
R-factor	0.16744
Rwork	0.165
R-free	0.21144
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.554
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	0.540
Number of reflections	70880
<I/σ(I)>	19.72	2.76
Completeness [%]	100.0	100
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	295	protein: 7.1 mg/mL in 10 mM Tris-HCl, pH 8.3, 250 mM sodium chloride, 5 mM BME, crystallized with the PACT Suite (B1: 0.1 M MIB buffer, pH 4, 25% w/v PEG1500), VAPOR DIFFUSION, SITTING DROP, temperature 295K