4GQD
DNA Holliday junction stabilized by chlorine halogen bond.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-10-21 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.692, 23.565, 37.286 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 30.680 - 1.940 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2org |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.680 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.228 | |
| Number of reflections | 3538 | |
| <I/σ(I)> | 3.25 | |
| Completeness [%] | 73.8 | 54.2 |
| Redundancy | 1.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.7mM DNA, 25mM sodium cacodylate pH 7.0 buffer, 10-25mM calcium chloride, and 0.8-1.2mM spermine, equilibrated against a reservoir of 30-40% aqueous MPD., VAPOR DIFFUSION, SITTING DROP, temperature 298K |
