4GQ9
Chikungunya virus neutralizing antibody 9.8B Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.859, 57.726, 118.108 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.995 |
| R-factor | 0.3251 |
| Rwork | 0.323 |
| R-free | 0.35930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dgg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.995 | 2.995 |
| Rmerge | 0.095 | 0.388 |
| Number of reflections | 11714 | |
| <I/σ(I)> | 22.5 | 4.3 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG 3350, 0.1M Tris-Cl pH 8.5 and 0.2M lithium sulfate., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
