4GQ2
S. pombe Nup120-Nup37 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.475, 123.019, 172.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.400 |
| R-factor | 0.21222 |
| Rwork | 0.210 |
| R-free | 0.25814 |
| Structure solution method | MR-SAD |
| Starting model (for MR) | 4gq1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.910 |
| Number of reflections | 56356 | |
| <I/σ(I)> | 34 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 303 | 10% PEG3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M ammonium acetate, EVAPORATION, temperature 303K |
