4GPW

Crystal structure of the protozoal cytoplasmic ribosomal decoding site in complex with 6'-hydroxysisomicin (P21212 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	33.791, 92.293, 47.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 3.000
Rwork	0.209
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.124
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHENIX
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.200
High resolution limit [Å]	3.000
Number of reflections	3263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Na Cacodylate, Spermine, NaCl, MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K