4GPO
Oligomeic Turkey Beta1-Adrenergic G Protein-Coupled Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.660, 79.590, 69.040 |
| Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
| Resolution | 29.779 - 3.500 |
| R-factor | 0.3121 |
| Rwork | 0.310 |
| R-free | 0.35460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y04 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 3.440 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.141 | |
| Number of reflections | 17463 | |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20mM sodium acetate, 100-300mM ammonium sulfate, 26~30% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
