4GPL
Structure of Cbl(TKB) bound to a phosphorylated pentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 122.574, 122.574, 56.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.660 - 3.000 |
| R-factor | 0.18549 |
| Rwork | 0.182 |
| R-free | 0.24828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cbl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.685 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.660 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.161 | 0.442 |
| Number of reflections | 38058 | |
| <I/σ(I)> | 6.4 | 2.4 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 3.93 | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.25M potassium sodium tartrate tetrahydrate, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
