4GPA

High resolution structure of the GluA4 N-terminal domain (NTD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9393
Spacegroup name	P 43 21 2
Unit cell lengths	104.540, 104.540, 108.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.860 - 2.250
R-factor	0.18928
Rwork	0.187
R-free	0.23491
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.664
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	54.490
High resolution limit [Å]	2.250
Number of reflections	29299
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.74M sodium citrate, 0.1M cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K