4GP6
Polynucleotide kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 130 |
| Detector technology | PIXEL |
| Collection date | 2012-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.690, 71.560, 118.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.780 - 2.100 |
| R-factor | 0.181 |
| Rwork | 0.177 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22214 | |
| Completeness [%] | 98.9 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES, 200mM MgCl2, 30% PEG 400, 2mM ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
