4GOT
Crystal structure of a putative methionine-binding lipoprotein (BSU32730) from Bacillus subtilis subsp. subtilis str. 168 at 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.440, 39.130, 55.663 |
| Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
| Resolution | 44.561 - 1.950 |
| R-factor | 0.1747 |
| Rwork | 0.172 |
| R-free | 0.22860 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.602 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (March 15, 2012) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.561 | 2.020 | |
| High resolution limit [Å] | 1.950 | 4.190 | 1.950 |
| Rmerge | 0.102 | 0.061 | 0.588 |
| Number of reflections | 16953 | 1952 | 935 |
| <I/σ(I)> | 11.54 | 24.6 | 2.2 |
| Completeness [%] | 89.9 | 98.3 | 50.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 40.0% polyethylene glycol 400, 0.2M lithium sulfate, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
