4GOP

Structure and Conformational Change of a Replication Protein A Heterotrimer Bound to ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Wavelength(s)	1.04022
Spacegroup name	C 1 2 1
Unit cell lengths	184.100, 93.800, 120.500
Unit cell angles	90.00, 110.90, 90.00

Refinement procedure

Resolution	20.000 - 3.100
R-factor	0.2441
Rwork	0.242
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.363
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.210
High resolution limit [Å]	3.100	6.660	3.100
Rmerge	0.103	0.043	0.512
Number of reflections	32477
<I/σ(I)>	7.7
Completeness [%]	92.3	86.9	94.5
Redundancy	2.7	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1