4GO7
The regulatory subunit of aspartate kinase in complex with threonine from Mycobacterium tuberculosis
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | I 41 |
| Unit cell lengths | 62.371, 62.371, 137.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.790 - 2.000 |
| R-factor | 0.22115 |
| Rwork | 0.219 |
| R-free | 0.26425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.074 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17555 | |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 1.8M ammonium phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
