4GO6
Crystal structure of HCF-1 self-association sequence 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.899, 183.512, 86.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.700 |
| Rwork | 0.231 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.081 | 0.637 |
| Number of reflections | 42424 | |
| <I/σ(I)> | 31.3 | 3.88 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 2.23M ammonium sulfate, 5% Isopropanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
