4GNX

Structure of U. maydis Replication protein A bound to ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	78.100, 91.400, 114.600
Unit cell angles	90.00, 97.80, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.2217
Rwork	0.219
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.331
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.068	0.025	0.574
Number of reflections	37317
<I/σ(I)>	11.6
Completeness [%]	94.5	87.5	97.4
Redundancy	2.4	2.4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1