4GNP
Structure of rat cytosolic PEPCK Ld_3g in complex with PEP and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.875, 84.612, 118.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.740 - 1.744 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dtb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.810 |
| High resolution limit [Å] | 1.744 | 3.770 | 1.744 |
| Rmerge | 0.062 | 0.031 | 0.499 |
| Number of reflections | 61576 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 97.6 | 90.7 | 98.9 |
| Redundancy | 5 | 5.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 22-26% PEG3350, 0.1 M HEPES, pH 7.4, 10 mM manganese chloride, 10 mM GDP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
