4GNA
mouse SMP30/GNL-xylitol complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-05-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.967, 101.967, 147.548 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.616 - 1.850 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gnb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.620 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 76106 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.6M ammonium sulfate, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
