4GMR
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR266.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.114, 43.592, 69.942 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 42.852 - 2.377 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XEE chain A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.377 | 2.400 |
| Rmerge | 0.138 | 0.568 |
| Number of reflections | 24422 | |
| <I/σ(I)> | 7.98 | 1.4 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | 40% PEG 1000, 0.1M potassium nitrate, 0.1M HEPES, pH7.5, Microbatch crystallization under oil, 291K |
