4GMQ
Ribosome-binding domain of Zuo1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0665 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 29.880, 44.060, 66.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.263 - 1.300 |
| R-factor | 0.1532 |
| Rwork | 0.151 |
| R-free | 0.18800 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.887 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.263 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.064 | 0.296 |
| Number of reflections | 22243 | |
| <I/σ(I)> | 15.7 | 5.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2.4 M ammonium sulfate, 0.1 M Tris, pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
