4GML
Crystal structure of human NOT1 MIF4G domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.270, 133.823, 331.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.310 - 2.900 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gmj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.706 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.089 | 0.774 |
| Number of reflections | 44067 | |
| <I/σ(I)> | 17.8 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M BTP, 1.6M AmSO4, 0.2M LiCl , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
