4GMK
Crystal Structure of Ribose 5-Phosphate Isomerase from the Probiotic Bacterium Lactobacillus salivarius UCC118
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-28 |
| Detector | PSI PILATUS 2M |
| Wavelength(s) | 0.917 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.340, 63.800, 59.840 |
| Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.720 |
| R-factor | 0.17149 |
| Rwork | 0.170 |
| R-free | 0.20596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.929 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.540 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 48927 | |
| Completeness [%] | 99.2 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.8M NaH2PO4/1.2M K2HPO4, 0.1M acetate, pH4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
