4GM2
The crystal structure of a peptidase from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.898, 102.162, 96.414 |
| Unit cell angles | 90.00, 114.53, 90.00 |
Refinement procedure
| Resolution | 48.790 - 2.800 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.051 | 0.028 | 0.755 |
| Number of reflections | 39735 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 99.8 | 97.1 | 100 |
| Redundancy | 3.8 | 3.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TrisHCl, 20% (v/v) ethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
