4GLW
DNA ligase A in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | Mar225 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 56.730, 56.730, 197.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.410 - 2.000 |
| R-factor | 0.20938 |
| Rwork | 0.207 |
| R-free | 0.25994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | INTEGRATE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.410 | 39.410 | 2.200 |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.119 | 0.053 | 0.430 |
| Number of reflections | 48105 | 386 | 11976 |
| <I/σ(I)> | 8.51 | 19.07 | 3.37 |
| Completeness [%] | 99.9 | 99 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.6 M sodium formiate, 0.1 M Hepes, 36 % (w/v) PEG 4000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
