4GLP
The crystal structure of soluble human CD14 reveals a bent solenoid with a hydrophobic amino-terminal pocket.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 147.520, 147.520, 44.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.879 - 4.002 |
| R-factor | 0.285 |
| Rwork | 0.283 |
| R-free | 0.32110 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.310 | 4.100 | |
| High resolution limit [Å] | 4.000 | 17.890 | 4.000 |
| Rmerge | 0.086 | 0.056 | 0.842 |
| Number of reflections | 4780 | 49 | 341 |
| <I/σ(I)> | 17.2 | 29.35 | 3.19 |
| Completeness [%] | 100.0 | 74.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 30% PEG 6000, 0.1 M 2-(N-morpholino)ethanesulfonic acid, 0.2 M calcium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
